FEI Vitrobot Mark III

Equipment Location: 
MRL B59

The FEI Vitrobot (Vitrification Robot) Mark III (Type FP 5350/61) is a fully PC-controlled cryo-plunger for vitrification (= rapid cooling) of aqueous samples used later in cryo-electron microscopy (typically in JEOL JEM-2100 TEM). The process of plunging, blotting and vitrification is fully automated and uses liquid ethane. It performs cryo-fixation under constant physical and mechanical conditions (temperature, relative humidity, blotting conditions and freezing velocity). This ensures reproducible high quality cryo-fixation results and sample preparation throughput for the following cryo-TEM observation. Subsequently, the specimens are typically transferred to liquid nitrogen environment and placed into Cryo-TEM-holder (Gatan CT 3500). The Controller (Gatan ITC 502S) will allow temperature setting down to approx. -185 oC.

FEI Vitrobot Mark III