TA Instruments Affinity ITC

TA Instruments Affinity ITC

Isothermal Titration Calorimetry (ITC) measures the heat evolved or absorbed during a binding reaction occurring in a liquid medium – typically water. Microliter amounts of titrant are incrementally added to the analyte solution in the reaction cell and the heat of reaction is measured at each step until saturation. Software is then implemented to fit the reaction curve to known or developed chemical reaction models in order to calculate parameters such as binding constant (K_a), stoichiometry (n), enthalpy (ΔH) and entropy (ΔS). Features of the Affinity ITC are listed below.

Cell – Cylindrical, 24k Gold, 1mL volume

Syringe – AccuShot™ injection syringe, 0.01 µL minimum injection volume, up to 250 µL total volume

Mixer – FlexSpin™ stirrer, 0 – 200 rpm

Temperature range – 2 °C – 80 °C

Detectable energy range – 0.1 µJ – 5000 µJ